Journal: BMC Cancer

Article Title: Identification of pyroptosis-associated miRNAs in the immunoscape and prognosis of hepatocellular carcinoma

doi: 10.1186/s12885-024-13276-5

Figure Lengend Snippet: Differential expression of pyroptosis-related miRNAs in HCC and normal tissues. ( A ) Volcano plot showing differentially expressed miRNAs (DEmiRNAs) in normal (N) and tumor (T) tissues. Red dots represent up-regulated miRNAs, and green dots represent down-regulated miRNAs. ( B ) Heatmap displaying the top 20 differentially expressed miRNAs between HCC and normal tissues

Article Snippet: Extracted RNA was reverse transcribed with the StarScript III miRNA RT Kit (by stem-loop) (A237, GenStar, Beijing, China) to obtain cDNA. qRT-PCR is applied with the ABI Prism 7700 Sequence Detection System, steps as follows: 95° C for 5 min, 40 cycles of 95° C for 30 s, 60° C for 45 s, and 72° C for 90 s and 72° C for 10 min.

Techniques: Expressing

Journal: BMC Cancer

Article Title: Identification of pyroptosis-associated miRNAs in the immunoscape and prognosis of hepatocellular carcinoma

doi: 10.1186/s12885-024-13276-5

Figure Lengend Snippet: Construction of risk scores for prognostic models based on pyroptosis-related miRNAs. ( A ) Univariate Cox regression analysis used to identify 10 prognosis-related miRNAs. ( B ) Forest plot summarizing the results of multivariate Cox regression analysis. ( C-D ) Least absolute shrinkage and selection operator (LASSO) regression analysis for selecting the optimal miRNAs associated with overall survival

Article Snippet: Extracted RNA was reverse transcribed with the StarScript III miRNA RT Kit (by stem-loop) (A237, GenStar, Beijing, China) to obtain cDNA. qRT-PCR is applied with the ABI Prism 7700 Sequence Detection System, steps as follows: 95° C for 5 min, 40 cycles of 95° C for 30 s, 60° C for 45 s, and 72° C for 90 s and 72° C for 10 min.

Techniques: Selection

Journal: BMC Cancer

Article Title: Identification of pyroptosis-associated miRNAs in the immunoscape and prognosis of hepatocellular carcinoma

doi: 10.1186/s12885-024-13276-5

Figure Lengend Snippet: Validation of the pyroptosis-related miRNA prognostic model. ( A ) PCA plot of HCC patients grouped by risk scores. ( B ) Distribution of risk scores for the patient cohort. ( C ) Survival status distribution of patients. ( D ) Kaplan-Meier survival curves comparing low-risk and high-risk groups. ( E ) ROC curves for predicting 1-, 3-, and 5-year overall survival. ROC analysis was used to calculate AUC values. ( F ) Calibration curves for 1-, 3-, and 5-year overall survival. (Number of High-Risk Samples: 183. Number of Low-Risk Samples: 183)

Article Snippet: Extracted RNA was reverse transcribed with the StarScript III miRNA RT Kit (by stem-loop) (A237, GenStar, Beijing, China) to obtain cDNA. qRT-PCR is applied with the ABI Prism 7700 Sequence Detection System, steps as follows: 95° C for 5 min, 40 cycles of 95° C for 30 s, 60° C for 45 s, and 72° C for 90 s and 72° C for 10 min.

Techniques: